Nyssomyia intermedia is an important vector of American cutaneous leishmaniasis. Methods: Sand flies were captured in a Shannon trap and after landing on human collectors and retrieved monthly. Nocturnal activity was estimated using a log-linear model. Results: Peak sand fly collection in the Shannon trap occurred in summer and winter, and peak fly collection on humans occurred in spring and summer. Conclusions: Ny. intermedia was captured in both the hottest and coldest months, indicating its adaptability to different seasons and the possibility of disease transmission at any time of the year in Campus Fiocruz Mata Atlântica, Brazil.