Introduction: The National Reference Laboratory (LRN) in Tuberculosis (TB) is a laboratory unit of highly specialized technical excellence that has developed projects involving training in human resources and the enhancement of techniques essential for the diagnosis of TB. Its purpose is the continuous improvement of all laboratory processes and their quality management, with the purpose of collaborating on and strengthening the actions in the network that integrate it in the aid of surveillance and in the fight against TB. Objectives: This research aims to describe the quality indicators that were defined through the process mapping methodology, with the objective of promoting and raising the level of quality and reliability of the different laboratory processes performed at LRN in TB. Method: For the definition of indicators, process mapping was used which, through flow charts, can establish and determine the most appropriate indicators for LRN in TB. After defining these indicators, they were described with their respective titles, preventive and corrective measures, importance and goals. Results: The results of the research suggest that the established indicators are fundamental for the laboratory processes, since they improve quality management. Conclusion: Through this study, we seek to encourage and impel the managers involved in the field of TB and in the field of health on the utility and the need to control and measure processes, being able to standardize them with the help of indicators or other quality tools.